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The sections were ex- Tion. Conjugated antisera were absorbed with one Quarter volume of mouse liver powder before use. Specificity of staining for mink y- globulin was estab- Lished by absorbing conjugated antisera with isolated With unconjugated anti- mink- y- globulin antisera fol- Lowed by staining with conjugated anti- mink- y- globu- Normal and AD infected mink showed anti- y- globulin Factor activity when tested with cells tanned with vari- Ous species y- globulins.
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The Tanned cell agglutination reactions observed between Mink sera and cells tanned with various heterologous Species y- globulins could be inhibited by mink aggre- Gated y- globulins but not by mink y- globulin cleared Table I. Agglutination and Inhibition Reactions Noted With Normal and AD Mink Sera Tested No. ulin gates ulin uHn ulin In most instances the factors reacting with both mink Aggregated y- globulin tanned cells and heterologous Fractions of sucrose density gradients.
Occasional The macroglobulin character of these mink anti- y- Globulin factors was confirmed in both normal and AD Infected animals, and in the serum of the same animal Sera before and after infection with AD Harley davidson touring foot pegs that After AD infection had become established. No Marked increase in IgM in AD animals was notable When normal and AD mink serum were separated Into electrophoretic components by zone electropho- Resis on starch, isolated mink y- globulins were obtained.
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